Benutzerspezifische Werkzeuge

NMR-Spektroskopie (NMR)

Platzhalter Bild

Dr. Alexander Funk

Group Leader

Focus

Metabolomics is the characterization of the metabolome. Metabolites are small molecules like amino acids, organic acids. Metabolic measurements via Nuclear Magnetic Resonance (NMR) Spectroscopy allows high throughput screening of bodyfluids. A wide array of Metabolites can be measured with minimal sample preparation and using a ‘push-button’ analysis for Urine (up to 150 parameters) and Plasma/Serum samples (up to 42 parameters). Additionally, a Lipid-profile panel is available. The automated setup of the NMR at the IKL allows batch preparation of samples, as well as automated acquisition of spectra.

In addition to the analysis of the liquid samples, we are equipped with a probehead for investigations using semi-solid materials. This can include bone marrow, intact tissues from biopsies or even whole cells. Here, a metabolic profile can be created for each sample for direct comparison or simple quantification. Finally, tracer studies using 13C or 15N are also possible.

Specific expertise

  • Large scale studies possible for most biofluids
  • Creation of automated metabolic profiles for Urine and Plasma/Serum
  • Analysis of semi-solid materials

 

Lab Members

Dr Funk, Alexander Group leader
Jonas, Sophie Technician

Selected Publications

  • Funk, A. M.; Wen, X.; Hever, T.; Maptue, N. R.; Khemtong, C.; Sherry, A. D.; Malloy, C. R. Effects of Deuteration on Transamination and Oxidation of Hyperpolarized C-13-Pyruvate in the Isolated Heart. J. Magn. Reson. 2019, 301, 102–108.
  • Courtney, K. D.; Bezwada, D.; Mashimo, T.; Pichumani, K.; Vemireddy, V.; Funk, A. M.; Wimberly, J.; McNeil, S. S.; Kapur, P.; Lotan, Y.; Margulis, V.; Cadeddu, J. A.; Pedrosa, I.; DeBerardinis, R. J.; Malloy, C. R.; Bachoo, R. M.; Maher, E. A. Isotope Tracing of Human Clear Cell Renal Cell Carcinomas Demonstrates Suppressed Glucose Oxidation In Vivo. Cell Metab. 2018, 28 (5), 793.
  • Maptue, N.; Jiang, W.; Harrison, C.; Funk, A. M.; Sharma, G.; Malloy, C. R.; Sherry, D.; Khemtong, C. Esterase-Catalyzed Production of Hyperpolarized C-13-Enriched Carbon Dioxide in Tissues for Measuring PH.ACS Sens. 2018, 3 (11), 2232–2236.
  • Imakura, Y.; Nonaka, H.; Takakusagi, Y.; Ichikawa, K.; Maptue, N. R.; Funk, A. M.; Khemtong, C.; Sando, S. Rational Design of [C-13,D-14]Tert-Butylbenzene as a Scaffold Structure for Designing Long-Lived Hyperpolarized C-13 Probes. Chem.-Asian J. 2018, 13 (3), 280–283.
  • Hashami, Z.; Martins, A. F.; Funk, A. M.; Jordan, V. C.; Petoud, S.; Eliseeva, S. V.; Kovacs, Z. COVER: Lanthanide DO3A-Tropone Complexes: Efficient Dual MR/NIR Imaging Probes in Aqueous Medium.Eur. J. Inorg. Chem.2017, No. 43, 4965–4968.
  • Funk, A. M.; Anderson, B. L.; Wen, X.; Hever, T.; Khemtong, C.; Kovacs, Z.; Sherry, A. D.; Malloy, C. R. The Rate of Lactate Production from Glucose in Hearts Is Not Altered by Per-Deuteration of Glucose. J. Magn. Reson. 2017, 284, 86–93.
  • Senanayake, P. K.; Rogers, N. J.; Finney, K.-L. N. A.; Harvey, P.; Funk, A. M.; Wilson, J. I.; O’Hogain, D.; Maxwell, R.; Parker, D.; Blamire, A. M. A New Paramagnetically Shifted Imaging Probe for MRI. Magn. Reson. Med.2017, 77 (3), 1307–1317.
  • Zhang, L.; Martins, A. F.; Mai, Y.; Zhao, P.; Funk, A. M.; Jordan, M. V. C.; Zhang, S.; Chen, W.; Wu, Y.; Sherry, A. D. Imaging Extracellular Lactate In Vitro and In Vivo Using CEST MRI and a Paramagnetic Shift Reagent.Chem.-Eur. J. 2017, 23 (8), 1752–1756.
  • Funk, A. M.; Jordan, V. C.; Sherry, A. D.; Ratnakar, S. J.; Kovacs, Z. Oxidative Conversion of a Europium(II)-Based T-1 Agent into a Europium(III)-Based ParaCEST Agent That Can Be Detected In Vivo by Magnetic Resonance Imaging. Angew. Chem.-Int. Edit. 2016, 55 (16), 5024–5027
  • Funk, A. M.; Harvey, P.; Finney, K.-L. N. A.; Fox, M. A.; Kenwright, A. M.; Rogers, N. J.; Senanayake, P. K.; Parker, D. Challenging Lanthanide Relaxation Theory: Erbium and Thulium Complexes That Show NMR Relaxation Rates Faster than Dysprosium and Terbium Analogues.Phys. Chem. Chem. Phys. 2015, 17 (25), 16507–16511
  • Funk, A. M.; Finney, K.-L. N. A.; Harvey, P.; Kenwright, A. M.; Neil, E. R.; Rogers, N. J.; Senanayake, P. K.; Parker, D. Critical Analysis of the Limitations of Bleaney’s Theory of Magnetic Anisotropy in Paramagnetic Lanthanide Coordination Complexes.Chem. Sci. 2015, 6 (3), 1655–1662.
  • Funk, A. M.; Fries, P. H.; Harvey, P.; Kenwright, A. M.; Parker, D. Experimental Measurement and Theoretical Assessment of Fast Lanthanide Electronic Relaxation in Solution with Four Series of Isostructural Complexes.J. Phys. Chem. A 2013, 117 (5), 905–917. 
  • Walton, J. W.; Carr, R.; Evans, N. H.; Funk, A. M.; Kenwright, A. M.; Parker, D.; Yufit, D. S.; Botta, M.; De Pinto, S.; Wong, K.-L. Isostructural Series of Nine-Coordinate Chiral Lanthanide Complexes Based on Triazacyclononane.Inorg. Chem. 2012, 51 (15), 8042–8056.